Our new Obvious+ tissue clearing method is the only real strategy that delipidates samples without any alter in morphology and with negligible influence on structural integrity.
Megatome can be a vibrating microtome created to part a wide array of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With higher blade vibrating frequency and minimized blade deflection, Megatome permits substantial-throughput tissue sectioning with uniform surface profile, together with nominal tissue harm and knowledge reduction.
Antibodies may perhaps get months to diffuse by means of only some millimeters of tissue, using a steep labeling gradient from area to core.
Megatome is suitable for accuracy: the blade vibrates at an increased frequency and larger amplitude vary than other microtomes, and includes a distinctive deflection Regulate system.
SE employs a rotational electrical area to disperse extremely electromobile molecules (including antibodies or surfactant micelles) during a porous sample without detrimental electrically billed constructions inside the tissue. This enables two-four working day clearing of intact organs,
Our preformulated EasyIndex Option raises and homogenize the refractive index of delipidated tissue samples, rendering them thoroughly clear. This enables light penetration into your sample and makes certain the acquisition of large-resolution, in-concentrate image info.
You should only really need to established the javadocTask home in the event the process you employ to create Javadoc is non-typical. As an example, there may be a undertaking referred to as allJavadoc in a multi-module
It’s time for you to enhance your microtome to Megatome. With correct superior-frequency slicing for an unmatched variety of sample measurements and types – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous apps.
Totally delipidate full mouse brains or comparably sized samples in only one working day with SmartBatch+, or in a single 7 days with our passive clearing kit.
Docset generation needs at least two choices: the title of the docset and The placement of the Javadoc information to incorporate within the docset.
This may deliver a feed Listing in the javadoc2dash.outputLocation Listing. This Listing will include an XML file describing the feed
Defend avoids the variability of hydrogel embedding and the data loss from PFA preservation, defending specimens for many rounds of processing.
--displayName: Will set the title as revealed in Dash. This can be useful for those who create a docset with name SampleProject but Screen identify Sample Project rather. This megatomi.com placing will default to the worth of --identify if omitted.
Our novel Protect tissue preservation system forms intramolecular bonds utilizing polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.